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KMID : 0387720140250030218
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Korean Journal of Blood Transfusion
2014 Volume.25 No. 3 p.218 ~ p.225
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Evaluation of the Automated Blood Bank Instrument QWALYS-3 for Cross-Matching Tests
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고영은:Koh Young-Eun
윤정:Yoon Jeong/권선희:Kwon Sun-Hee/김윤호:Kim Yoon-Ho/최재열:Choi Jae-Yeol/김주연:Kim Joo-Yeon/조치현:Cho Chi-Hyun/임채승:Lim Chae-Seung/윤수영:Yoon Soo-Young
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Abstract
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Background: The cross-matching test is regarded as an essential pre-transfusion test. It serves an important role in confirming the ABO/Rh compatibility of transfusion and screening for possible unexpected antibodies. We evaluated cross-matching tests in QWALYS-3 (DIAGAST, Loos Cedex, France), comparing the automated process to manual tube methods.
Methods: A total of 545 crossmatching tests from 169 patients, collected from RBC concentrate transfusion orders, were performed using both QWALYS-3 and manual tube methods. All patients were follow-up tested later on with antibody identification tests to confirm the presence of unexpected antibodies in plasma.
Results: None of the samples were ABO/Rh incompatible. The presence of unexpected antibodies was later confirmed in 277 tests in 56 patients. Out of those 277 tests, the concordance rate between two methods was 83.8% (232/277). In 268 tests which were later confirmed with no unexpected antibodies, manual tube methods did not show any positive results while five tests were false-positive (5/268, 1.9%) only in QWALYS-3. The overall concordance rate between two methods was 90.82%, and the kappa coefficient was 0.696 (P＜0.05) (n=545).
Conclusion: The QWALYS-3 system has its merits in accuracy, precision, and lack of possible human errors, however, the automated procedure showed some disadvantages, including relatively low cost-and-time-effectiveness, less effective cold antibody detection, and difficulties in handling small quantity samples. Thus, the QWALYS-3 system has meaningful, but only a limited value in the automation of routine cross-matching tests.
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KEYWORD
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Blood bank, Cross-matching, Automation, QWALYS-3
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